Lab 2a:
dissecting a cell and examining its components.
purpose: What are positive indicator tests for proteins, carbohydrates, and fats?
Do parts of an egg test positive for protein, carbohydrate, and or fat?
Materials list:
eggs tubes, glass, 13x100mm gelatin
beaker, 250ml peg racks for 13x100mm tubes sodium hydroxidewhite vinegar glucose (dextrose) cupric sulfate 5- hydrate oilplastic wrap benedict's solution sudan IV solution
slotted spoon hot plate stirrer salpel handles, #4
sodium chloride test tube holder scapel blades, #22 for #4 handles
distilled water starch, soluble trays, plasticbeaker, 100ml lugol's iodine solutionpipets, 5ml vortex mixer
pipet pump, green
Procedure: (part 1)
1. Place an uncooked egg in vnegar and leave it there for 24 to 48 hours while the shell desolves.
2. After time is up gentally pick up the egg with a slotted spoon, use water to rise of the vinegar. You will see a chemical action happen here. Which chemicals were used as reactants and products of this reaction.
3. Feel the sells outer membrane, it is thinner, flexible, and permeable to some substances. In order to make sure that water can go in and out of the cell put it in NaCL solution for 24 hours.
4. Gently cut open the eggs membrane and drain the egg white into a 100ml beaker without loosing or breaking the yolk inside.
5. Put the yolk into a separate beaker and keep the membrane to the side.
procedure: (part 2)
procedure: (part 3)
1. Substitute the egg for sugar, fat, starch, or protein. you will conduct the same experiment just with different materials.
2. Be sure to write down the results from the egg membrane, egg whites, and the yolk.
3. Make a numerical value for the test results you have received by using the system of the key also take note of any color changes that occur.
data and results:
Part 1:
CaCO3+CH3COOH = H2CO3 + H2O + CO2
Day 1
The egg was put into vinegar.
Day 2
The shell disolved and then we put the egg in sodium cloride. The salt went into the egg and the water went out.
Day 3
The egg we put the egg into deionized water. This put water back into the egg and the egg got bigger.
Day 4
We cut the egg open, the insides came out of the egg. All the water put the egg under pressure causing this to happen when we cut it open.
Part 2:
Do parts of an egg test positive for protein, carbohydrate, and or fat?
Materials list:
eggs tubes, glass, 13x100mm gelatin
beaker, 250ml peg racks for 13x100mm tubes sodium hydroxidewhite vinegar glucose (dextrose) cupric sulfate 5- hydrate oilplastic wrap benedict's solution sudan IV solution
slotted spoon hot plate stirrer salpel handles, #4
sodium chloride test tube holder scapel blades, #22 for #4 handles
distilled water starch, soluble trays, plasticbeaker, 100ml lugol's iodine solutionpipets, 5ml vortex mixer
pipet pump, green
Procedure: (part 1)
1. Place an uncooked egg in vnegar and leave it there for 24 to 48 hours while the shell desolves.
2. After time is up gentally pick up the egg with a slotted spoon, use water to rise of the vinegar. You will see a chemical action happen here. Which chemicals were used as reactants and products of this reaction.
3. Feel the sells outer membrane, it is thinner, flexible, and permeable to some substances. In order to make sure that water can go in and out of the cell put it in NaCL solution for 24 hours.
4. Gently cut open the eggs membrane and drain the egg white into a 100ml beaker without loosing or breaking the yolk inside.
5. Put the yolk into a separate beaker and keep the membrane to the side.
procedure: (part 2)
- Mix 2 ml of 2% Glucose water with 2 ml of Benedict's solution. Then heat the mixture for 2 minutes in 250 ml of water at 100 degrees C. Make note of the color changes.
- Mix 2 ml of deionized water with 2 ml of Benedict's solution in a test tube. Heat it in the same beaker and heat as before. Once again make note of the color changes that happen.
- Mix 2 ml of startch suspension with 0.25 ml of Lugol's iodine. Softly mix these together. Caution: do not heat! Make not of color changes.
- Mix 2 ml of deionized water suspension with 0.25 ml of Lugol's iodine. Softly mix these together. Once again do not heat the solution! Make note of the color change.
- Put 2 ml of gelatin in a test tube and add 0.5 mL of 10% NaOH and mix, Add 0.25 mL of 5% copper sulfate and mix. while doing so wear goggles and gloves. Note the color changes.
- put 2 ml of deionized water in a test tube and add 0.5 of 10% NaOH and mix, Add 0.25 mL of 5% copper sulfate and mix. Wait 30 seconds and note the color change you observe.
- On a brown paper bag place a drop of oil, let the droplet dry for about 10 minutes and hold it to the light. Write down the amount of light that passes though the oil spot, in a percentage.
- on a brown paper bag place a drop of water on it, let the droplet dry for about 10 minutes and hold it to the light.Write down the amount of light that passes though the oil spot, in a percentage.
procedure: (part 3)
1. Substitute the egg for sugar, fat, starch, or protein. you will conduct the same experiment just with different materials.
2. Be sure to write down the results from the egg membrane, egg whites, and the yolk.
3. Make a numerical value for the test results you have received by using the system of the key also take note of any color changes that occur.
data and results:
Part 1:
CaCO3+CH3COOH = H2CO3 + H2O + CO2
Day 1
The egg was put into vinegar.
Day 2
The shell disolved and then we put the egg in sodium cloride. The salt went into the egg and the water went out.
Day 3
The egg we put the egg into deionized water. This put water back into the egg and the egg got bigger.
Day 4
We cut the egg open, the insides came out of the egg. All the water put the egg under pressure causing this to happen when we cut it open.
Part 2:
Part 3:
I really liked examining the egg after it got bigger in the solution because, it felt like a water balloon and was really cool to touch. I really enjoyed this experiment and hope to do more like it this year, I really liked working with Ronan but we don't really get that much done so I will have to work on staying on task and being focused when I work with him again. over all this project was really fun I learned a lot about chemicals and what they can do, but I do want to start getting into the bigger stuff because, I am a little more interested in it then the molecules.